Relationship between transformation in Bacillus subtilis and infection by bacteriophage SP02.
نویسندگان
چکیده
When bacteriophage SP02 infects a Bacillus subtilis culture during or shortly after transformation, the frequency of transformants among the resulting lysogens is greatly reduced relative to that in the uninfected culture. The effect can occur after the deoxyribonucleic acid has been taken up and covalently attached to recipient deoxyribonucleic acid.
منابع مشابه
Characterization of interspecific plasmid transfer mediated by Bacillus subtilis temperate bacteriophage SP02.
Plasmid pPL1010 is a 7.0-kilobase derivative of plasmid pUB110 that harbors the cohesive end site of the bacteriophage SP02 genome. Plasmid pPL1017 is a 6.8-kilobase derivative of plasmid pC194 that contains the immunity region of bacteriophage phi 105 and the cohesive end site of bacteriophage SP02. These plasmids are transducible by bacteriophage SP02 at a frequency of 10(-2) transductants pe...
متن کاملCorrelation of the Genetic Map and the Endonuclease Site Map of Bacillus subtilis Bacteriophage SP02.
By marker rescue of bacteriophage SP02 sus mutants with purified bacteriophage SP02 DNA fragments, 11 of the 17 known bacteriophage SP02 sus loci were assigned to discrete DNA fragments. The left-most genetic locus, susA, was found to reside near one bacteriophage SP02 terminus (EcoRI-C1 fragment), whereas the right-most genetic locus, susP, was found to reside near the other bacteriophage SP02...
متن کاملHigh-frequency elimination of SP02 prophage from Bacillus subtilis by plasmid transformation.
Transformation of competent Bacillus subtilis lysogenic for SP02 with any of three plasmids (pCM194, pUB110, pAM77) generates drug-resistant transformants of which 5 to 20% have lost the infectivity and immunity associated with the SP02 prophage. Such cured derivatives can be again lysogenized with SP02 and again cured by introduction of a different plasmid. Elimination of the SP02 prophage was...
متن کاملIsolation and properties of suppressor-sensitive mutants of Bacillus subtilis bacteriophage SP02.
Suppressor-sensitive mutants of Bacillus subtilis phage SPO2 were isolated and classified into 18 cistrons. A linear genetic map was constructed. The genes controlling early functions of phage development were clustered near the CI gene which may produce phage repressor.
متن کاملEvidence that Bacillus subtilis bacteriophage SP02 is temperate and heteroimmune to bacteriophage phi-105.
Some characteristics of Bacillus subtilis phage SPO2 which show that it is a temperate phage are presented. Wild-type SPO2 forms turbid plaques, similar to those of other temperate phages. SPO2 lysogenic strains which are resistant to SPO2 can be isolated; these strains remain stable lysogens despite the fact that they can no longer adsorb SPO2. SPO2 lysogenic strains can be grown for many gene...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 116 2 شماره
صفحات -
تاریخ انتشار 1973